Bio-Rad Laboratories, a manufacturer and distributor of life-science research and clinical diagnostic products, has launched its Precision Melt Analysis software.
This software enables high-resolution-melt (HRM) analysis on Bio-Rad's CFX96 and CFX384 real-time PCR systems.
HRM extends the usefulness of quantitative real-time PCR methodology to detailed post-PCR sequence analysis, enabling a range of applications, such as mutation scanning, methylation analysis and SNP genotyping.
With its simplicity and abbreviated workflow, HRM analysis offers a cost-effective yet accurate alternative to other post-PCR genetic analysis techniques, such as single-strand conformation polymorphism (SSCP), mass spectrometry and sequencing.
Precision Melt Analysis imports and analyses data files generated from Bio-Rad's CFX96 or CFX384 real-time PCR detection systems.
Researchers can use the software to genotype samples based on the thermal denaturation properties of double-stranded DNA.
They can also scan for new gene variants, screen DNA samples for SNPs, identify insertions/deletions or other unknown mutations and determine the percentage of methylated DNA in unknown samples.
The software works with the CFX96 and CFX384 systems to deliver sensitive and reliable detection for HRM applications.
This package accommodates the needs of all HRM users, whether they are performing a single genotyping experiment or analysing a comprehensive study comprised of multiple runs, according to the company.
The software allows the user to: streamline data analysis using the customisable default analysis settings; use multiple data view options to manually assign sample genotypes by tailoring the software to the appropriate analysis; view multiple charts at once (with the 'Charts' view, the user can check all of the data charts, including the amplification plot); and increase analysis throughput (users can analyse multiple experiments from several melt runs together using the 'Melt Study' feature).
