Cygel Sustain from Biostatus is designed to simplify zebrafish embryo imaging for microscopy, high-content screening and site-specific micro-injections, even from a few hours post-fertilisation.
This thermo-reversible mountant is a liquid when cooled below room temperature and sets to a clear, optically inert gel when warmed.
Cygel Sustain accepts embryo media and anaesthetics such as MS-222, when needed, to maintain yet completely immobilise dechorionated embryos.
After imaging, embryos can be recovered with excellent viability even after one hour in Cygel Sustain, permitting observation of further development stages.
Cygel Sustain can also be used for the mounting of fixed embryos.
Biostatus said that Cygel avoids all the problems associated with methylcellulose and low-melting point agarose.
