Using Cygel from Biostatus, C.elegans worms can now be held still for live whole organism imaging and site-specific micro-injection of drugs and SiRNA.
Worms can be immobilised for up to one hour and then gently recovered to feed, develop and/or produce embryos.
Cygel accepts the M9 buffer and the helminth-specific anaesthetic levamisole.
Cygel is a liquid that can be dispensed from a pipette when cooled below room temperature.
It forms an optically inert, clear gel mountant when warmed, suitable for both phase contrast and fluorescence microscopy.
This mounting method is said to be much simpler and may open up new possibilities for microscopy and HCS applications of C.elegans.
