Viscotek has published application notes illustrating the extended capabilities of gel permeation chromatography/size exclusion chromatography (GPC/SEC) using the PDA detector for the TDAMax system.
Available on the Viscotek website, these application notes illustrate how the detection technology brings greater flexibility to multi-component characterisation and analysis in fields from polymer science to biopharmaceuticals.
The application notes describe 'Conjugated polymer analysis by GPC-PDA' and 'Polymer blend analysis by GPC-PDA' respectively.
Both are downloadable.
The Viscotek TDAmax is a comprehensive GPC/SEC system with an integrated triple or tetra detector array.
By capturing absorption spectra across the UV-vis wavelength range, the PDA detector provides a fingerprint of each 'time slice' of the sample as it elutes from the column.
Measurement times are 30-40 minutes.
UV detection is used routinely to identify protein and polymer species as they elute, and can differentiate between components when other detection methods cannot.
The PDA detector captures data across a 190-500nm range, allowing users to select wavelengths of interest after the analysis has been carried out.
The information it gives can be used in both product and process development.
The UV cell of the PDA sits in the temperature controlled zone of the TDAMax, operating at up to 80C.
Viscotek's OmniSEC software package allows data to be displayed as information-rich 3D images that simplify interpretation.
When GPC/SEC is coupled with detectors, a range of additional parameters can be determined, including intrinsic viscosity, molecular size, and long-chain branching.
