Biologists can now use the functions of the Zeiss Axiovision software, such as the enhancement of fluorescence signals and the suppression of image noise, to support their Fret investigations.
These features are found in the physiology module of the Axiovision 4.7 microscopy software and are said to make Fret experiments simpler.
The Fret software is used to determine the energy transfer portion between two adjacent protein molecules through fluorescence energy transfer (Fret), measure the distance between adjacent protein molecules below the microscope resolution and obtain quantitative temporal and spatial information about the bonding and interaction between proteins, lipids, enzymes, DNA and RNA in vivo.
Carl Zeiss said it is ideally matched to the motorised Axio Imager or Cell Observer microscope systems, although it can be to be used with manual fluorescence microscopes.
The software offers additions to existing and new methods of Fret measurement by using different corrective techniques or acceptor bleaching.
Users can define their own formulas for the tasks to be performed or use pre-programmed Fret formulas to make Fret experiments simpler.
Furthermore, it is possible to modify existing Fret formulas and create new evaluation techniques via the formula field.
Axiovision enables the range of microscope accessories and software functions to be used for processing.
For instance, up to 140 frames per second are available with the Axiocam HS and the dual camera module more than doubles the speed of capture.
In addition, all required time series images (donor, acceptor and Fret) can be captured using the physiology module.