The active layer is made up of reactive epoxy groups that form an immediate and irreversible covalent bond with all the probe's nucleophilic groups, without UV cross-linking or reduction
Schott Nexterion is a manufacturer of microarray slides and has consistently built on its application expertise since launching the first commercially available epoxy slide more than three years ago.
Many scientists already reap the benefits of this active surface chemistry that facilitates the immobilisation of oligonucleotides.
Schott Nexterion offers future users a proactive and competent consultancy service for all application queries.
In this way, customers have access to the experience gathered by the market pioneer.
In addition, for first-time users there is a starter kit with optimised reagents for spotting and hybridisation.
The active layer is made up of reactive epoxy groups that form an immediate and irreversible covalent bond with all the probe's nucleophilic groups, without requiring UV cross-linking or reduction.
An amino modification of the oligonucleotide is only necessary when spotting short oligonucleotides (ó 25mers).
Nexterion Slide E also makes the spotting of small spots possible and consequently high-density microarrays with regular and reproducible spot morphology.
The surface chemistry is stable and remains active even during long spotting runs.
For the spotting of PCR products, Schott Nexterion offers slides with aminosilane and aldehyde coatings.
The aminosilane slides, Nexterion Slide A and Nexterion Slide A+ afford the user particulary flexible application possibilities. Using the same protocol, PCR molecules and even longer oligonucleotides (ò 50 mers) can be spotted on this aminosilane surface.
As both slide types also work with common PCR protocols, users have a problem-free changeover to these Schott Nexterion slides, without requiring the time-consuming re-optimisation of the assay process.
All Schott Nexterion coated slides are manufactured using a special borosilicate glass.
The low inherent fluorescence of the glass substrate together with the homogenous coating of the different slide types guarantee excellent sensitivity and uniform, optimised spots in microarray experiments.
