Integrated DNA Technologies (IDT) has announced a number of advancements to its MIRcat small RNA cloning system.
The updated kit is now faster, permitting the cloning of small regulatory RNAs (MiRNAs, PiRNAs, SiRNAs and so on) from any cellular source in any species.
The kit now includes the Performa spin columns from Edge Biosystems.
Featuring a fully hydrated gel matrix, the columns provide a simple method for recovering extremely pure RNA from denaturing Page gels.
The IDT MIRcat small RNA cloning system is compatible with the majority of existing relevant laboratory protocols.
'By decreasing the overall protocol time and adding the Performa Spin Columns, the MIRcat kit now offers researchers a system for creating small RNA libraries quickly and consistently,' said Dr Mark Behlke, chief scientific officer at IDT.
Based upon a pre-activated adenylated oligonucleotide linkering method, the MIRcat cloning system consists of three sequential protocols: RNA isolation and enrichment, cloning linker attachment and amplification and cloning.
Specific primers are also available to convert the resultant small RNA libraries using Roche/454 Life Science sequencing systems.
