Bio-Rad Laboratories has released an instructional video that guides researchers that want to efficiently transfect their primary cells.
The educational video, entitled 'Using the Gene Pulser MXcell Electroporation System to Transfect Primary Cells With High Efficiency' is available on the company's website.
Bio-Rad's Gene Pulser MXcell electroporation system and Gene Pulser electroporation buffer, which emulates the composition of cell cytoplasm, were specifically designed to facilitate the transfer of nucleic acids into mammalian cells and difficult-to-transfect cells such as primary and stem cells.
By watching the video, researchers can learn how to perform a simple experiment to quickly identify the best electroporation conditions for a cell-line of interest.
The video also shows a shortcut that enables an experiment to be conducted simultaneously with optimisation.
As another convenient, time-saving measure, the video shows how scientists can interchange reaction vessels without re-optimising settings, making it possible to utilise the same conditions in electroporation plates or cuvettes.
An advantage of the Gene Pulser MXcell electroporation system is that, in addition to preset protocols, every critical parameter is programmable - from voltage to number of pulses - enabling researchers to find the ideal set of conditions for their specific cells, including difficult-to-transfect cells.
