Waters has highlighted its family of ultra-performance liquid chromatography (UPLC) solutions at Pittcon 2010, including the Acquity and Patrol systems.
Scientists from Astrazeneca and Eli Lilly presented real-life success stories working with the Waters Acquity UPLC system and Patrol UPLC system respectively, at Waters's annual Pittcon news conference.
The Acquity UPLC H-Class system is the newest addition to the company's UPLC family.
It is claimed to combine performance, simplicity and flexibility to help laboratories realise the scientific and business benefits of sub-2um (micron) particle column technology.
Beyond improving chromatographic performance when running UPLC columns as well as fully supporting HPLC columns, the Acquity UPLC H-Class system closely emulates HPLC workflows, making the method transfer from HPLC to UPLC easier.
Waters said the introduction of this system allows organisations to standardise their approach to LC with a common technology platform that makes the future transition from HPLC to UPLC-based methods straightforward and practical.
At Pittcon 2010, Waters is exhibiting an array of column chemistries for UPLC-based analytical solutions for the characterisation of biomolecules including glycoproteins, proteins and their aggregates, monoclonal antibodies and recombinant proteins.
For the characterisation of 2-AB labelled glycans from glycoproteins, Waters is combining its Acquity UPLC BEH glycan columns with the Acquity UPLC system equipped with fluorescence detection.
Food and Drug Administration (FDA) regulations require that firms developing and manufacturing therapeutic proteins are able to accurately characterise the glycans attached to those proteins to ensure the efficacy and safety of a biopharmaceutical product.
Waters is introducing this solution for scientists seeking to apply the power of UPLC technology to characterise the glycosylation of therapeutic proteins during their development.
For the analysis of intact biomolecules, including monoclonal antibodies and their aggregates, and recombinant proteins, Waters is introducing a family of Protein-Pak Hi Res Ion-Exchange (IEX) columns.
This column family consists of one anion-exchange - quaternary ammonium - column chemistry and two cation-exchange column chemistries - a carboxy methyl and a sulfopropyl - each contained on monodisperse, non-porous particles.
The multi-layered network of ion-exchange functional groups on the particles results in high sample loading capacities for proteins not found with the use of typical non-porous particle IEX offerings.
For separating and quantifying monoclonal antibodies (mAb) and their aggregates in less than four minutes, Waters is introducing the Acquity UPLC BEH200 1.7um SEC columns for the Acquity UPLC system.
The company said its Acquity UPLC SEC solution makes it faster and easier for manufacturers to achieve high-quality data that meets FDA requirements for quantifying protein aggregates suspected of being capable of producing an immunogenic response in certain patients.
Rounding out Waters's column offering, Viridis SFC columns are a family of analytical and preparative supercritical fluid chromatography (SFC) columns.
These columns were developed to give laboratories a reliable source of high-quality SFC columns for compound purification.
Paired with Waters SFC systems, and extending our full range of Waters's Optimum Bed Density (OBD) columns for HPLC systems, laboratories now have more choices for instrumentation and columns for their HPLC and SFC purification work.
Viridis columns are initially available in two chemistries: 2-ethylpyridine and silica.
Waters's Viridis SFC columns are manufactured by Waters ISO 9000 facilities under quality guidelines and feature the company's patented preparative OBD technology, designed to produce mechanically stable columns with longer lifetimes.
High-throughput chromatography laboratories are tasked with analysing large numbers of samples, each typically requiring off-line solid phase extraction (SPE).
Waters has introduced an alternative to this off-line SPE process by combining the benefits of UPLC separating power with on-line, automated SPE.
A UPLC-based system with on-line SPE technology can streamline the analysis of samples by providing analyte extraction, concentration, separation, and detection in one turnkey solution.
Automated sample handling, proprietary column chemistries, and ultra-sensitive mass spectrometry detection have been combined into an on-line SPE/LC/MS/MS solution that requires only a fraction of the typical sample volume while the sensitivity of the system exceeds the limits set by regulatory bodies.
