Bio-Rad Laboratories is collaborating with Layerlab to enable researchers to study the binding kinetics of any transmembrane protein using surface plasmon resonance (SPR) technology.
Using Memlayer, Layerlab's methodology and easy-to-use chemistry kit, researchers can immobilise liposomes containing any transmembrane protein to Bio-Rad's Proteon XPR36 protein interaction array system sensor chip surfaces for label-free high-throughput kinetic analysis with high signal quality.
The array-based Proteon XPR36 system is said to offer researchers the ability to speed their drug discovery workflow due to its capability to simultaneously monitor 36 interactions.
The combination of the Proteon XPR36 system and Memlayer accelerates and enhances membrane protein research, according to Bio-Rad.
Examining transmembrane proteins in liposomes, as opposed to isolated and bound to a sensor surface, enables researchers to mimic the cellular environment and preserve protein functionality.
Immobilising and capturing liposomes directly to the biosensor surface, however, can be challenging.
Some liposomes bind weakly with SPR biosensors.
Analysing the captured liposomes can be difficult since liposomes contain fewer proteins than a standard sensor surface, reducing signal quality.
The Memlayer tag is spontaneously incorporated into any liposome membrane, including those of synthetic lipoparticles available through Bio-Rad's collaboration with Integral Molecular, fractionated cell membranes, and cellular lipid vesicles such as microsomes and endosomes.
This allows for kinetic analysis of transmembrane proteins in liposomes that cannot be immobilised by standard techniques such as through Biotin-Neutravidin derivitisation.
Layerlab's liquid bilayer immobilisation method allows researchers to achieve previously impossible densities of membrane-bound receptor molecules.
Additional liposome-incorporated tags can link to other tagged liposomes, creating multiple layers of lipoparticles above the sensor surface.
These layers boost the density of receptor proteins and increase data quality.
Layerlab immobilisation method allows for continual regeneration of biosensor surfaces, therefore extending the lifetime and utility of Bio-Rad sensor chips.
The biosensor surface can be regenerated with readily available deionised water, which breaks apart the hybridised Memlayer tag pairs and washes away the previously attached liposomes.
Using Layerlab's Memlayer chemistry kit requires little hands-on time.
Incorporation of the Memlayer tag to any liposome in the laboratory requires a 15min incubation period at room temperature.
Immobilisation of the liposomes to the Proteon XPR36 sensor surface requires 10-20min.
The Proteon XPR36 protein interaction array system is a multiplexed SPR biosensor that allows users to simultaneously measure the interactions of six different ligand proteins with panels of six different concentrations of analyte, obtaining comprehensive kinetic profiles in a single experiment without the need for regeneration.
This is termed 'One-Shot Kinetics' technology.
Utilising an optical design and microfluidics, the Proteon XPR36 system is said to provide a higher throughput for rapid screening of protein interactions than traditional SPR devices.
Biologics, such as antibodies, are vital reagents in basic biomedical research and for the diagnosis and treatment of various diseases.
Screening, profiling, and characterising antibodies can be tedious and time-consuming.
The Proteon XPR36 system is said to reduce the amount of time required for screening targets - including transmembrane proteins which are often drug targets - as well as optimising assay design, and profiling and characterising antibody interactions.
According to Bio-Rad, the system is ideal for scientists conducting antibody research in drug discovery and development.
Key application areas for these customers include protein interface analysis, interaction proteomics, and drug target interactions.
