Bio-Rad Laboratories has launched Ssofast probes supermix, a reagent that enables researchers using fluorogenic probes to enhance the speed, reliability and sensitivity of qPCR experiments.
By combining Bio-Rad's Sso7d fusion polymerase with an optimised buffer formulation, the Ssofast probes supermix is said to yield fast duplex qPCR results in 30 minutes or less.
The company claimed that Ssofast probes supermix pushes the limits of fast qPCR, while maintaining a broad dynamic range of detection, reliability and accurate quantification.
In addition, the polymerase is more resistant to PCR inhibitors, making Ssofast probes supermix ideal for demanding qPCR applications.
Bio-Rad said researchers can incorporate the Ssofast probes supermix effortlessly into their real-time PCR experiments.
The supermix is fully compatible with universal cycling conditions and a range of primer/probe concentrations without requiring additional optimisation.
It can also be used on both fast and standard cycling instruments without protocol changes.
The performance of Ssofast probes supermix is enhanced when used with Bio-Rad's CFX96 or CFX384 real-time PCR systems.
The thermal uniformity and detection capabilities of the instrument take full advantage of the supermix's fast polymerase and optimised formulation to deliver fast and reproducible qPCR results.