Kronlab has used its custom-build approach to design a high-performance liquid chromatography (HPLC) system for peptide separations in pilot scale.
Often client-specific requirements for multipurpose equipment can not be solved with 'off-the-shelf' solutions.
In this specific example, every component has been specifically approved for the application required and following a specified procedure, it is integrated into an LC system.
The result is a made-to-measure system.
This example has been designed for method optimisation of peptide separations in pilot scale and the intended use in a potentially explosive atmosphere.
In addition to a binary high-pressure gradient pump and a 'metal-free' HPLC pump for sample application, modules for temperature, mass flow and pressure data acquisition were included.
A four-way, two-position valve enables the bypassing of the column.
A multiple wavelength UV detector ensures the detection of the target peptides.
The fractionation of up to 32 samples works with a well-thought-out valve assembly, which allows the purging of the fractionation tubing with nitrogen after fraction change and is not limited to a defined volume.
The choice of components and their placing in a nitrogen-pressurised cabinet allows work in areas with potentially explosive atmosphere to be undertaken safely.
In addition, Kronlab provides the customer with detailed documentation of testing as well as qualification or support for IQ/ OQ/PQ verification.
Individual, client-specific testing and acceptance criteria can be incorporated on request.
Maintenance and service is provided by qualified scientists or skilled engineers with experience of such systems.
