Integrated DNA Technologies (IDT) has signed a deal with 454 Life Sciences to provide researchers with custom primers for the GS FLX Titanium chemistry used on the GS FLX and GS Junior systems.
Available as Fusionprimers or Rapid Library MID (Molecular Identification) Adaptor Oligos, these products are used for amplicon or shotgun sequencing.
IDT's design software enables users to create specific primers to target individual or multiple exons from one or more genes.
Through the use of HPLC purification techniques, IDT reduces the potential occurrence of mis-priming and identity is ensured with 100 per cent mass spectrometry quality control.
John Havens, vice-president of business development at IDT, said: 'Applications within genomic sequencing are becoming increasingly prominent, especially with the wide use of next-generation sequencing.
'As such, we are delighted to enable researchers to use GS FLX Titanium Fusionprimers and Rapidlibrary MID Adaptors for 454 sequencing applications, from ultra-deep amplicon sequencing to transcriptome sequencing and metagenomics,' he added.
The GS FLX Titanium Fusionprimers have a 25 base-pair fixed sequence at the 5ft end and enable the sequencing of longer amplicons and increased reads per run.
An optional MID sequence acts as a molecular barcode to enable libraries to be multiplexed and subsequently identified.
The GS FLX Titanium Rapidlibrary MID Adaptor Oligos are intended for shotgun sequencing and incorporate MIDs as standard.
These adaptors are said to be useful for sequencing numerous small samples in parallel in order to drop the effective sequencing cost per samples.
The Standard Fusionprimers, the GS FLX Titanium Fusionprimers and the GS FLX Titanium Rapidlibrary MID Adaptor Oligos are all available to order via IDT's free online suite of design tools, called Scitools.
