Thermo Fisher is currently showcasing the Thermo Scientific Nunclon Vita stem cell culture surface at the Biotechnica exhibition, which runs until 7 October 2010.
According to the company, which will be found in Booth 9/G51 at the event in Hannover, Germany, the energy-treated polystyrene surface enables direct attachment, colony formation and the growth of human embryonic stem cells (ESCs) and induced pluripotent stem (IPS) cells without the need for feeder cells or matrix coatings.
In media supplemented with a Rho-associated kinase (Rock) inhibitor, human ESCs can be cultured on the Nunclon Vita surface for at least 10 passages without any loss of pluripotency or karyotype changes.
As a result, Nunclon Vita represents a step forward for stem cell scientists, offering greater control over the culturing of stem cells and providing a more stable supply of cells for downstream applications.
By eliminating matrix coatings and feeder cells, the Nunclon Vita surface reduces culture variability and is less labour intensive than cell lines that require matrix coatings or feeder cells.
The Nunclon Vita surface treatment is more adaptable for scalable cell expansion as cells grow directly on the polystyrene surface.
In addition, the new surface works in synergy with a Rock inhibitor for the expansion of human ESCs, IPS cells and human embryonic kidney (HEK) cells to enable enzymatic and mechanical passage protocols.
The Thermo Scientific Nunclon Vita surface is the latest addition to a series of cell culture surface products, including the Thermo Scientific Nunclon Delta surface, and joins a range of technologies and products for all basic and advanced cell-based research.
