Amsbio has introduced kits for the isolation of high-quality RNA from Formalin-Fixed Paraffin-Embedded (FFPE) tissues and LCM samples that can be applied to qRT-PCR and microarray platforms.
Expressart FFPE RNAready overcomes the limitations associated with RNA degradation and interference from RNA cross-linking in FFPE tissues.
The FFPE RNAready kit efficiently isolates and preserves mRNA, utilising a specially developed lysis solution and a universal inhibitor of RNases that displaces tightly bound RNA from high-molecular-weight aggregates.
Subsequent treatment with a de-modification reagent reverses formalin-induced cross-links, resulting in total RNA optimised for reverse transcription reactions and subsequent downstream applications.
Expressart FFPE RNAready TRinucleotide primer technology is now available to amplify microgram quantities of high-quality RNA from very small amounts of degraded total RNA, with minimal loss of sequence.
