Integrated DNA Technologies (IDT) has developed a quantitative real-time polymerase chain reaction (qPCR) user guide, which covers the basics of experimental set-up, performance and analysis.
The document provides user guidance on the entire qPCR process - from RNA isolation to data analysis.
Specific information on nuclease assays, including re-suspensions and qPCR protocols are also supplied, as well as a troubleshooting section that discusses commonly encountered issues.
The document is written in compliance with MIQE guidelines: minimum information for publication of quantitative real-time PCR1.
IDT offers a broad range of Primetime qPCR assay products including customised primers and probes, in addition to the free online design suite - Scitools.
