Agilent's Sureselect XT Mouse All Exon Kit is said to be the first commercial system for target enrichment of a model organism exome and is used to streamline next-generation sequencing experiments.
Agilent collaborated with Dr Yoichi Gondo of Japan's Riken Institute to develop the mouse exome target-enrichment product.
'We tested the product on our ENU-mutagenised mouse genome, in which one ENU-induced mutation was induced approximately every 1Mb,' said Dr Gondo.
'Using the Sureselect Mouse All Exon kit we found 61 ENU-induced mutations at the very first trial, which was much more than we expected,' he said.
Sureselect XT combines sequencing library preparation and gDNA preparative reagents with the Sureselect target-enrichment system.
The system has been cited in more than 25 papers, covering research into a range of heritable disorders.
Agilent's SureselectXT Target Enrichment System offers a range of complete target-enrichment solutions and optimised protocols for a range of sequencing methods and platforms.
Sureselect XT products are available for enrichment of target sizes ranging from smaller than 200Kb to more than 50Mb in a single tube.
Target enrichment streamlines workflows by letting researchers sequence just the genomic regions of interest rather than entire genomes.
Combined with the increasing capacity of next-generation sequencers, the multiplexing support capabilities of the Sureselect XT platform make it possible for geneticists to interrogate the genomes of more samples per study than previously possible.
One of the rate-limiting steps in such studies has been library preparation and target enrichment.
To enable high-throughput sample-processing efficiency, Agilent offers an integrated workstation that automates Sureselect XT library preparation and target-enrichment workflows.
Protocols now support Illumina end sequencing, paired-end sequencing and indexing protocols in addition to fragment library format, paired-end sequencing and barcoding on the Solid System.
Most recently, the Sureselect platform has been extended to the Roche 454 System, as well bringing the full range of customer-defined and catalogue Sureselect offerings to all three major next-generation sequencing instruments.
Sureselect XT also allows a high degree of customisation.
Users of Agilent's eArray xD desktop design tool can improve the efficiency of their research by easily designing custom products to target any genome of interest, all in a single tube.
Agilent also offers the eArray online design tool, enabling users to customise predefined Sureselect kits, such as the Sureselect Mouse All Exon series of products.
