Syngene's range of G:box Chemi image analysers can be used for imaging with infra-red Li-cor IRDye dyes, making it easier to detect and quantify different types of proteins on multiplex Western blots.
The G:box Chemi systems, when fitted with a combination of recommended lighting and specific Syngene filters, can be used for imaging Li-cor dyes, IRDye 680 (Epi Red Multiplex LED lighting module and Syngene 705M filter) and IRDye 800 (Epi LED IR 740 lighting module and Syngene LY800 filter).
The Genesys software in the G:box Chemi automatically selects the right lighting and filters for whichever IR dye or other fluorescent dyes scientists inform the system is on the blot.
The software then captures one perfect image of all the different dyes, to ensure imaging fluorescent multiplex Westerns is quick and simple.
Using fluorescence to visualise proteins on Western blots allows scientists to increase throughput by using the same blot to detect different proteins - something they can't do using chemiluminescent-based blots.
However it can be difficult to accurately quantify proteins as some fluorescent dyes have overlapping spectra and membranes can auto-fluoresce, which interferes with detecting low abundance proteins.
Using IR dyes can sometimes solve these problems, but detecting IR dyes can be difficult using CCD-based systems.
Syngene's G:box Chemi device offers a solution in delivering a sensitive, accurate method for imaging IR-labelled proteins, without having to buy an expensive laser-based scanner.
