In a recent study, Prokop et al used Roche's CASY Cell Counter and Analyzer to identify the specific anti-cancer properties of a therapeutic substance in Burkitt-like lymphoma cells (BJAB cells).
In addition to using the CASY system for multi-parameter quality control of the cell cultures, the team also brought the system to bear in examining apoptosis induction as early as 24 hours after treatment - before taking final measurements with Annexin-V and DNA fragmentation assays 24 and 48 hours later, respectively.
The research group headed by Aram Prokop at the Institute for Experimental Oncology of the Children's Hospital in Cologne, Germany, is studying new anti-leukemia and anti-tumour compounds that induce apoptosis in a range of human tumour cell lines and can overcome the multiple drug resistance in leukemia and tumour cells.
The group established leukemia and tumour cell lines that are resistant to the common cytostatics clinically used for treating children with acute lymphoblastic leukemia (ALL).
The team used various chemosensitivity assays to demonstrate the ability of newly synthesised compounds to overcome drug resistance in vitro (using drug-resistant leukemia cells) and ex vivo (using drug-resistant primary lymphoblasts from children with ALL).
The CASY Cell Counter and Analyzer improved the researchers' workflow in important ways: besides using the CASY system for cell culture quality control - for determining cell concentration, cell viability, cell volume, cell aggregation and cell debris - they also used it for studying apoptosis induction as early as 24 hours after treatment.
This saved 24 and 48 hours, respectively, when compared with Annexin-V and DNA fragmentation assays.
The CASY system accurately measures cell viability, but cannot differentiate between necrotic and apoptotic cells.
Combining CASY measurements with the LDH release assay (for early exclusion of nonspecific necrosis) clearly showed a correlation between decreasing cell proliferation, decreasing cell viability and the induction of apoptosis.
