A team of scientists recently conducted a study using the Xcelligence System from Roche Diagnostics, providing extensive information about the effects of extraterrestrial lunar dusts on human health.
The team, headed by Klaudia Brix, an expert in cell biology and skin regeneration at Jacobs University in Bremen, evaluated the effects of extraterrestrial dusts on mammalian skin.
Hacat keratinocytes and CHO-K1 fibroblasts were exposed for various periods of time to dusts that simulate lunar or Martian soils.
Particular attention was paid to changes in cell morphology and cell viability.
Keratinocytes and fibroblasts were used because human skin protects the human body from potentially harmful substances and is also where wound-healing occurs.
Real-time, label-free cytotoxicity measurements were made by continually monitoring cell impedance using the Xcelligence System and performing the Roche MTT assay.
In addition, phalloidin staining of the actin cytoskeleton and propidium iodide uptake assays were performed to investigate cellular structural integrity.
By looking at various functions of the two cell lines, the researchers were able to identify two types of simulated lunar dust.
Following exposure to the simulated lunar dust, Hacat keratinocyte proliferation, analysed using Ki67 labelling, was suppressed in sub-confluent cultures and regeneration of keratinocyte monolayers after a scratch wound was also found to be delayed.
In particular, the finding that extraterrestrial dust exposure has a negative effect on wound healing will result in additional risk-assessment studies when planning lunar lander extraterrestrial exploration.
Roche claims that these results will help scientists organise appropriate safety measures that will minimise the risks to human health when human skin is exposed to lunar or Martian dust on extraterrestrial missions.
