Bioo Scientific has launched the Nextflex small RNA sequencing kit, which is designed for next-generation sequencing library preparation.
The kit is said to provide a flexible and cost-effective solution for generating single and multiplexed libraries from small RNA using Illumina GAIIz and Hiseq 2000 sequencing platforms.
The Nextflex small RNA sequencing kit allows users to generate libraries directly from as little as 1ug of total RNA.
It contains a specialised 3' adenylated adapter that specifically ligates onto microRNAs and other small RNAs containing a 3' hydroxyl group.
After adapter ligation and reverse transcription, libraries are amplified using a barcoded primer, allowing up to 48 samples to be multiplexed and sequence variations interrogated.
The Nextflex small RNA sequencing kit incorporates Air Ligase, a truncated T4 RNA ligase, which increases the efficiency with which small RNAs are tagged with adapter, giving greater sequence depth.
The protocol is designed to reduce adapter dimmer formation prevalent in other protocols.
Strand specificity is retained through the use of adapters that ligate to the 5'-phosphate and 3'-hydroxyl groups that most mature miRNAs possess.
The kit can be used for mRNA directional sequencing.
In combination with the Nextflex small RNA barcode primer kits, 48 samples can be profiled and studied simultaneously.
Four sets of 12 Nextflex small RNA barcode primers, with embedded index sequences are available offering an improved multiplexing workflow and increased flexibility.
The automation-friendly format enables multiplexing of up to 384 samples.
The ability to pool samples in an efficient way decreases hands on time while providing robust data quality.
The primer barcoding system utilises a 6nt index to differentiate up to 48 different samples on a single flow-cell lane.
In addition to small RNA sequencing, the Nextflex kit is compatible with mRNA directional, RIP-Seq and Clip-Seq protocols.
