Fortebio has launched its Dip and Read Anti-Mouse IgG Fc Capture (AMC) biosensor for use on its Octet instrumentation platform.
The biosensor enables high-throughput rapid kinetic characterisation and screening of mouse antibodies and other Fc-containing proteins with ease of use and cost efficiency, according to the company.
Christopher Silva, vice-president of marketing at Fortebio, said: 'With the introduction of our AMC biosensor, researchers can benefit from our off-the-shelf, label-free biosensors during discovery projects using murine models, as well as when working with human antibodies.
'Our AMC biosensor eliminates the need for inconvenient, labour-intensive steps typically associated with ELISA and SPR methods for screening and kinetic characterisation, and thereby facilitates high-throughput research applications such as hybridoma screening and characterisation,' he added.
The AMC biosensor enables immobilisation of mouse Fc-containing proteins upon the biosensor directly, from a crude or purified matrix, without the need to biotinylate.
In addition, AMC biosensors can be regenerated and reused up to 10 times.
Silva said: 'Our AMC biosensor can also work fluidly with Fortebio's other research tools for increased speed, ease of use and cost-efficiency.
'For example, researchers using our Protein G biosensor to titer hybridoma samples can now use the AMC biosensor to perform kinetics and affinity analysis on resulting epitopes of interest - all from the same crude sample,' he added.
Fortebio's Anti-Human IgG Fc Capture biosensor is ready to use on the company's Octet instruments.
The Octet platform is based on Fortebio's Biolayer Interferometry (BLI) technology, which uses optical biosensors to measure multiple interactions in parallel, without the use of detection agents.
The platform consists of eight- and 16-channel instruments that accommodate 96- and 384-well assay formats.
All Octet biosensors are designed to simplify kinetic characterisation and quantitation by eliminating throughput and time-to-result limitations of traditional SPR-based assays and ELISA.
The Octet biosensors are disposable, configured in a standard microplate format and are coated with a proprietary biocompatible matrix that is uniform, non-denaturing and has minimal non-specific binding.
