Bioo Scientific's Nextflex-96 DNA Barcodes use bar-coded indices to run multiple samples in parallel.
According to Bioo Scientific, the ability to increase the scale of next generation sequencing is a critical requirement needed to make personalised sequencing a reality, and multiplexing is a big step in that direction.
The Nextflex-96 DNA Barcodes allow users to pool multiple library preparations in a single flow cell lane.
They accomplish this by providing 96 indexed adapters, each of which contains a unique 8nt sequence.
The indices are designed to be error resistant and allow for proper differentiation between samples.
Using the Nextflex-96 DNA Barcodes, the index is added to the sample during the adapter ligation step.
This allows PCR amplification to be reduced or eliminated and therefore minimises amplification-based bias and prevents poor reads from single base errors introduced during PCR.
These barcodes can be used to multiplex genomic DNA, RNA and ChIP sequencing libraries.
The Nextflex-96 DNA Barcodes have been optimised for use with the Nextflex DNA Sequencing Kit but can also be used with other Illumina library prep protocols.
Each set of barcodes has been functionally validated with Illumina GAIIx and Hiseq 2000 sequencing platforms.
For people who want to multiplex but do not need 96 barcodes, Bioo Scientific offers flexible options with the Nextflex DNA Barcodes, which are supplied in sets of six, 12, 24, or 48 unique barcodes.
