Bio-Rad Laboratories has launched its Immun-Blot LF (low fluorescence) PVDF membranes, protein blotting membranes that are optimised for fluorescence and multiplex fluorescence applications.
The membranes offer high signal-to-background ratio, low autofluorescence and superior protein retention to maximise blot detection sensitivity and enable downstream quantitation.
Two primary types of protein blotting membranes are used in western blotting applications: nitrocellulose and PVDF (polyvinylidene fluoride).
While both types are suitable for colorimetric and chemiluminescent detection, their high autofluorescence and lower signal-to-background ratios are not suitable for fluorescent detection.
Immun-Blot LF PVDF membranes are a low-fluorescence alternative specifically designed for fluorescent immunoblotting and detection in the visible light (red/green/blue) spectrum.
Immun-Blot LF PVDF membranes produce highly sensitive and quantitative results thanks to their superior signal-to-background ratios, which are three times higher than those of nitrocellulose and conventional PVDF.
Immun-Blot LF PVDF membranes are compatible with any imager possessing visible RGB excitation channels, including Bio-Rad's new Chemidoc MP imager, which allows researchers to visualise proteins at every stage of a blotting experiment when paired with Bio-Rad's proprietary Stain-Free technology.
The membranes are also compatible with a range of fluorescent labels, such as Dylights, Q-Dots and Alexa fluorophores, as well as colorimetric and chemiluminescent detection methods.
