Multimode unit has enabled scientists to develop a high throughput system for reporter-based drug screening assays in living zebrafish disease models1.
Tecan's Infinite M1000 multimode microplate reader has enabled scientists in the Department of Cellular Biology and Anatomy at Georgia Health Sciences University (GHSU), USA, to develop a high-throughput system for reporter-based drug screening assays in living zebrafish disease models1.
Quantitative microplate reader systems have revolutionised the pace of drug discovery, enabling the development of reporter -based in vitro and in silico assays that allow high throughput screening (HTS), explains Dr Jeff Mumm, Assistant Professor at GHSU.
However, he noted, biological validation has become a bottleneck in the drug discovery process, due to a lack of HTS-compatible in vivo assay platforms.
"Quantifying reporter levels in living zebrafish provides a versatile means of alleviating this road block," said Mumm.
"The flexibility of the Infinite M1000 has allowed us to develop a simple and cost-effective automated screening method based on fluorescent or luminescent reporter detection in live zebrafish".
Using this method, GHSU researchers have been able to quantify the loss and regeneration of targeted cells in zebrafish disease models, as well as small molecule-induced changes in disease-linked molecular signaling pathways.
"High signal to noise ratios allow us to monitor changes in individual fish - which accounts for wide reporter level variance across populations by normalizing signals to each individual's ground state - enabling us to detect long-term changes over several days," said Mumm.
The technique, he forecast will benefit "a wide variety of high throughput chemical and genetic screens in live zebrafish, potentially accelerating the validation of new drugs."
