Dolomite has developed a tool for the encapsulation of single cells into droplets for use in screening and droplet PCR.
The system provides users with the tools required to perform assays on individual cells in picolitre volume environments.
The technology was demonstrated by feeding 2.8µm diameter magnetic beads into monodisperse 100µm diameter droplets at a ratio of 1 bead per droplet with minimal bead losses.
The Mitos P-Pump allows stirring of the input fluid reservoir, to provide an even distribution of beads and delivers a pulseless and stable flow for excellent control of droplet volumes.
Leak-free connections are made from pump to tube to chip using Dolomite’s Multiflux Linear connectors.
Droplets are isolated in an inert fluorocarbon carrier stream to prevent cross contamination and are stabilised with Pico-Surf, a biocompatible surfactant designed specifically for generating pico-droplets.
