MALDIVision facilitates data processing of individual ions across the sampled locations on a tissue section.
MALDI IMS, an acronym for Matrix Assisted Laser Desorption Ionisation Imaging Mass Spectrometry, is a new technology for visualising the spatial distribution of analytes.
It has been increasingly used for candidate biomarker discovery, drug metabolite profiling, lipid analysis and proteomics.
MALDI tissue imaging contributes significantly towards disease diagnosis and is now being used to address a number of clinical questions in diseases such as cancer and organ development.
However, to apply MALDI Imaging technology for clinical purposes, it is essential to develop a computationally powerful data processing and visualisation informatics tool which can aid in successful analysis of the large data files obtained during an experimental run.
MALDIVision, a comprehensive bioinformatics tool for MALDI tissue imaging, facilitates data processing, visualisation and analysis of spatial distribution of individual ions across the sampled locations on a tissue section.
The program accepts imaging mass spectrometric data in standard file formats. MALDIVision generates an ion intensity map for any m/z in the analysis
Using the software:
-Users can view images in 2-D and in 3-D. MALDIVision also facilitates generation of Extracted Ion Image (EII) for specific mass peaks extracted directly from the mass spectrum.
-Users can display the reconstituted image, the EII as well as the optical image in different color gradients, so as investigate the localisation or distribution of compounds present in a sample.
-The region of biological significance can be marked to observe the ion intensity distribution. MALDIVision enables scrolling through the m/z peaks of a mass spectrum which in turn facilitates in analyzing spatial distribution of peptides, lipids and metabolites in tissue compartments.
-Users can generate a histogram and a cumulative probability graph to observe the ion intensity distribution in the defined ROI.
range, enabling simultaneous imaging of many compounds.
The program also calculates the statistics such as mean, median, standard deviation for the region of interest chosen and exports them for lab records.
