The FluoView FV1200 claims to provide sensitive live cell imaging capabilities using less laser power.
The FluoView FV1200 has been designed for researchers who need more sensitive live cell imaging with high-speed measurement capability.
With its ultra-high sensitivity and innovative fluorescence measurement, it requires less laser power, resulting in lower phototoxicity and photobleaching.
Building on Olympus’ optical design capability, the FV1200 offers increased laser selection, cooled high-sensitivity detector technology, increased light throughput and faster functional measurements.
The system capitalises on the advantages of the company’s recently introduced IX83 automated inverted microscope platform.
Among its improvements for live cell imaging, the FV1200’s scanhead features increased sensitivity via its breakthrough anti-corrosive silver-coated galvo mirrors, which increase light throughput significantly over competitive instruments.
The entire system is optimised for sensitivity, with increased excitation and emission transmission efficiencies, particularly in the near-infrared (IR) light range, where much of today’s key in vivo and live cell imaging research occurs.
The FV1200 also offers a 748nm diode laser for researchers doing near-IR imaging.
Further increasing sensitivity is an optional cooled gallium arsenide phosphide (GaAsP) detector unit that minimizes electrical noise.
By using selected GaAsP detectors that deliver 45-percent quantum efficiency, users will experience some of the highest signal-to-noise imaging ratios possible.
When the GaAsP unit is used with three conventional confocal detectors, the FV1200 can acquire up to five simultaneous fluorescent channels with its near-IR 748nm laser diode, allowing users to image DAPI, GFP, RPF, Cy5 and Cy7 simultaneously.
Combining GaAsP and conventional photomultiplier tubes in one system allows users to select either higher-sensitivity or higher-dynamic-range imaging, providing important flexibility for imaging live cells.
The system’s multipoint mapping advanced software (MMASW) delivers functional measurements of high-speed calcium fluctuations in groups of cells at speeds up to 101 Hz per field, with sequential position data output up to 50,000 Hz.
The MMASW’s mapping feature allows pixel-skipping image generation in a field of fluctuating cells; it also provides automatic region-of-interest identification for further high-speed measurement.
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