The latest version of NextGENe software comes with CNV analysis module and customised reporting capabilities.
SoftGenetics has announced the release of the latest version of its NextGENe software.
Improvements include a module for Copy Number Variation (CNV) analysis and a user selectable reporting capability.
The software uses a proprietary method for normalising coverage between two samples- a list of heterozygous SNPs with allele percentages close to 50% is generated for each sample, and the median coverage for these alleles is used to normalise two samples.
After normalisation, a representative position is selected for each region and used to calculatea log2 ratio and a confidence score.
When selecting a position for comparison, a heterozygous mutation in one sample is preferred so that any allele frequency changes can be used to examine potential loss of heterozygosity, similar to existing CNV methods.
The user interface requires no scripting, provides highly graphical results and reporting with multiple filtering options, multiple analysis comparison capabilities as well as causative prediction from multiple databases.
