The NEXTflex rapid DNA-seq kit is designed to offer two hour NGS library construction from 1ng of DNA.
The kit, which enables researchers to build sequencing-ready libraries, is for use with genomic DNA, FFPE samples, ChIP DNA and also clinical samples.
It can be used between 1nd and 1µg of input data and the bead-based, gel-free size selection protocol is meant to eliminate the need for agarose gels.
Additionally, the availability of up to 96 unique adapter barcodes facilitates high-throughput applications.
The system incorporates ‘enhanced adapter litigation technology’ which aids the litigation of long adapters, resulting in more diverse sequencing reads.
According to Dr. Masoud Toloue, director of genomic research at Bioo, “The NEXTflex kit offers the perfect balance of simplicity and speed without sacrificing library fidelity, achieved by optimising the enzymatic reactions to allow multiple steps to be run simultaneously.”