The profile of the YSI Life Science’s 2900 series of biochemistry analysers continues to be raised following the publication of a number of research papers that have helped underscore just how important the instruments have now become to so many research efforts.
The authors of one recent paper which appeared in the journal Nature have successfully used a YSI 2900 system to evaluate glucose and lactate concentrations in 96 well plates as part of a study of KRAS-expressing cells.
KRAS mutations are known to be a driver event of pancreatic ductal adenocarcinomas (PDAC) one of the deadliest cancers in western countries.
Elsewhere, another important study in the field of oncology recently used a YSI 2900 series analyser in a study of anti-tumour activity of the glutaminase inhibitor CB-839 in triple-negative breast cancer.
This was reported in the journal Molecular Cancer Therapeutics.
For experiments quantifying metabolite consumption or production in tissue culture media the authors incubated their cells in Dulbecco’s modified Eagle media (DMEM) with 5 mmol/L glucose and 0.5 mmol/L glutamine (no serum) for six hours.
Media concentrations of glucose, lactate, glutamine, and glutamate were then quantified using the YSI 2900 Biochemistry Analyser.
Thanks to YSI’s proprietary immobilised enzyme technology it is now possible to simultaneously measure compatible ’chemistries’ from a very wide range of analytes including: ammonium, glutamine, glutamate, glucose, dextrose, sucrose, xylose, lactate, lactose, methanol, ethanol and hydrogen peroxide.
Laboratories using cell-culture’s - like oncology labs - were actually among the first to appreciate the benefits of the YSI approach to biochemical analysis, as it enables researchers to speedily monitor culture nutrients and quickly adjust their levels if necessary.
This facility has now been taken to a new level by YSI with the YSI 2900M Online Monitoring and Control system.
This has combined proven 2900-analyser immobilised enzyme technology, along with the YSI Sitini Online Sampler, to automatically allow the withdrawal of sample fluids from a user’s experiment or process.
The result is continuous feedback on the status of culture carried out automatically, and aseptically, with antiseptic lines flushed after each sample is withdrawn.
The YSI 2900M Online Monitoring and Control system can then talk to process management instrumentation via Ethernet, RS232, or OPC connections, for complete control over your experimental system.
The number of applications for YSI Life Science’s 2900 series of biochemistry analysers is expanding rapidly, to include everything from oncology to biofuel production.
More and more researchers and technologists are beginning to see the benefits of YSI’s fast (under one minute) precise and easy-to-use method of biochemical analysis, applicable for a constantly expanding number of chemistries.
