A study in the potential of online bioprocess monitoring featuring the use of a YSI Analyser and Flowmatics Seg-Flow
Written by Flownamics in collaboration with Scarab Genomics, the study published in GEN (Genetic Engineering & Biotechnology News) was conducted using E.Coli cultures where growth was controlled by glucose adjustment during the fed-batch phase, in order to prevent overflow metabolism.
Glucose utilisation and cell densities were monitored throughout a 25 hour period.
Glucose levels were determined using the YSI Analyser, after being drawn by the Seg-Flow at hourly intervals to provide real-time analysis.
Increased pressure to deliver to market sooner means industry trends are moving towards automated bioprocess monitoring in order to compress process development times.
The study successfully demonstrated that by controlling growth rates, overflow metabolism can be prevented and thus productivity increased, process development accelerated and ultimately drug development costs cut.