Cleaver Scientific has announced the introduction of a horizontal polyacrylamide gel electrophoresis (hPAGE) system developed in collaboration with the Kirkhouse Trust, an organisation that supports research and education in the biological sciences.
While vertical systems are commonly used for polyacrylamide gel electrophoresis, this unit’s horizontal format allows for a large number of samples to be run simultaneously and combines this with the greater resolution of polyacrylamide compared with agarose.
Developers say the main advantage of the system is that it is simple to pour and cast the polyacrylamide gels, which takes away some of the complexity normally associated with vertical gel casting.
Markers based on SSRs (simple sequence repeats) have been developed with the aim of generating elite lines with resistance to pathogens/parasites.
The Cleaver Scientific hPAGE system makes it possible to compare many samples and achieve a resolution of a 4-6 bp difference between markers, helping to distinguish between the resistant and non-resistant plants.