New chemistry extends application to fermentation of Pichia pastoris yeast cultures, the organism of choice for producing recombinant proteins
A new chemistry - methanol - has been added to the YSI 7100 MBS, available from Analytical Technologies, extending its application to fermentation of Pichia pastoris yeast cultures, the organism of choice for producing recombinant proteins.
Ensuring optimum levels of nutrients in cell fermentation media is critical to achieve maximum yields.
Pichia pastoris is sensitive to concentrations of methanol which need to be maintained within a narrow range.
As well as being necessary to control induction and regulation of the promoter for the desired gene expression, methanol also serves as a carbon source during the growth phase. However, high levels of methanol are cytotoxic and the accumulation of the metabolite formaldehyde must also be controlled.
With quick and accurate measurements available using the YSI 7100, methanol levels can be monitored to ensure they remain at optimum levels for high protein yield without becoming cytotoxic.
The SmartSet reagent management system, featuring barcode-labelled reagents and fluid-level detection, together with YSI's patented immobilised enzyme technology using mix-and-match colour-coded membranes, provide an efficient, user-friendly operation.
Information can be stored and accessed instantly for precise configuration of reagents and sensors with complete traceability.
Capable of simultaneous measurement of six distinct parameters, the YSI 7100 offers glucose, lactate, glutamate, glutamine, ammonium, potassium, sucrose and ethanol for a wide range of applications.
