New surface plasmon resonance chemistries for easier biomolecular binding analysis in membrane receptor studies, biomaterial research, and advanced kinetic analyses
Biacore International has announced the availability of five new sensor chip products with novel chemistries and a Thiol coupling kit for its surface plasmon resonance (SPR) technology. Increasing experimental flexibility and ease-of-use, the sensor chips and Thiol coupling kit will allow researchers to immobilise and study a wide range of biological entities, ranging from single peptide molecules to liposomes, viruses and even whole cells.
Adding to an already comprehensive range of Biacore sensor chips, these new surface chemistries, based on the previously released Pioneer series, increase the applications of SPR in areas such as membrane receptor studies, biomaterial research and advanced kinetic analyses.
Sensor chip CM4 reduces non-specific binding of positively charged molecules, as may be found in cell culture supernatants or cell homogenates and an ideal choice for ligand fishing and protein-DNA binding applications.
Sensor chip CM3 offers reduced steric effects when working with high molecular weight molecules, including cells and virus particles. Sensor chip C1, for work with particles such as whole cells and viruses, and in applications where a dextran matrix is not required.
Sensor chip Au, a plain gold surface that enables users to define customised surface chemistries using self-assembled monolayers or other modifications.
Sensor chip L1 for direct, rapid and reproducible capture of lipid membrane vesicles such as liposomes, without requiring incorporation of anchoring molecules.
The Thiol coupling kit allows ligands without amino groups to be immobilised.
Proteins or peptides with a single free cysteine can be immobilised in a directed way.
Reagents and coupling solutions for performing 50 surface thiol immobilisations, 18 ligand thiols or 22 PDEA ligand modifications are included.
Biacore continues to develop new sensor chip and reagent products for the growing applications of its SPR technology, proven by over 2900 references in the peer reviewed scientific press.
The wide range of sensor chips, combined with unique microfluidics technologies, enable the detailed study of binding kinetics, affinity, specificity and active concentration for a wide range of interactions.
