Bio-Rad has expanded its Immun-Star line of chemiluminescent kits for western blot detection to include the Immun-Star HRP kit for horseradish peroxidase (HRP) conjugated secondary antibodies.
Bio-Rad has expanded its Immun-Star line of chemiluminescent kits for western blot detection to include the Immun-Star HRP kit for horseradish peroxidase (HRP) conjugated secondary antibodies. This highly-sensitive, fast-acting alternative to isotopic detection is able to detect less than one picogram of sample, and the light signal lasts 6-8 hours after activation.
Successful experiments can be run with small amounts of sample, and there's no need to rush into the darkroom after activation. Working solutions are stable at room temperature for 24 hours, and kitsû shelf life is one year at 4deg C.
Film exposures are as low as 30 seconds to 2 minutes on commonly used blotting supports.
These superior light signals also produce excellent, quantifiable results on imaging systems such as Bio-Rad's ChemiDoc and VersaDoc systems.
The chemical reaction of Immun-Star HRP involves a peroxidase-catalyzed oxidation of luminol which produces light.
The addition of an enhancer, which is included in the kit, intensifies the light signal and increases its duration.
If desired, most blots can be reused by stripping and reprobing.
Immun-Star HRP kits are available in configurations to suit customer's precise needs, including: substrate only, substrate with conjugate and substrate with conjugate and blotting reagents.
