Rapid ligation kit can be used to ligate either blunt or cohesive ends, making it ideal for a range of applications
Cambridge BioScience has added CloneDirect rapid ligation kits from Lucigen to its range of cloning systems.
CloneDirect is a simple, highly efficient alternative to traditional DNA ligation protocols, requiring no DNA clean-up step prior to electroporation and ligation.
The kit can be used to ligate either blunt or cohesive ends making it ideal for a range of applications including shotgun library construction, cloning of blunt or cohesive end restriction fragments, PCR cloning, cDNA cloning and linker ligation.
Ligation using CloneDirect is fast, taking just five minutes for cohesive ends and 30 minutes for blunt ends.
High efficiency reactions give over 95% ligation of cohesive ends when using properly prepared DNA.
The procedure is simple and convenient to use - just add DNA and ligase directly to the premixed reagents.
With CloneDirect there is no tedious clean-up step following ligation.
Simply heat the ligation mix for 15 minutes at 70C to kill the ligation reaction and add directly to competent cells for transformation.
