State-of-the-art technology helps decode the RNA of the virus implicated in the worldwide outbreak of severe acute respiratory syndrome (Sars)
Using state-of-the-art laboratory automation and advanced molecular techniques, scientists at the BC Cancer Agency's Michael Smith Genome Sciences Centre in Canada have completed the first publicly available draft sequence for a corona virus implicated in severe acute respiratory syndrome (Sars).
Sars is a novel form of corona virus that can infect humans and cause severe influenza like symptoms, causing death in around 4% of cases [latest reports suggest mortality may be as high as 20% -Ed].
After receiving one-millionth of a gram of purified viral genetic material, scientists at the Genome Sciences Centre took the genetic material (RNA) and copied into a DNA form before inserting that into a bacteria in order to amplify the sequences for analysis.
The bacteria containing the Sars sequences were then picked using QPix automated colony picking systems supplied by Genetix and the DNA analysed.
Using this state-of-the-art automated equipment the Sars virus sequence (around 30,000 bases) was determined very rapidly, in around six days.
The sequence will allow diagnostic tests to be developed so that infected patients can be rapidly recognised and treated in an appropriate manner.
In the longer term, these results may allow scientists to understand why the Sars virus is so deadly, and may assist in the development of an effective vaccine.
