Colony pickers can be used in conjunction with isothermal DNA amplification method for swift, efficient sequence identity
Genetix has announced that its QBot or QPix2 colony pickers can be used in conjunction with Templiphi, a novel isothermal DNA amplification method developed by Amersham Biosciences, for swift, efficient sequence identity.
The fast, accurate and reproducible Genetix colony pickers combined with the capability of the Templiphi to prepare DNA of sequencing template quality directly from bacterial cells carrying plasmids results in reduced labour and consumable costs, saves valuable lab space and decreases the time taken from colony growth to sequence identity. Colony picking robots usually sterilise the picking pins between colony picking using ethanol baths.
This eliminates carryover of live cells but may not eliminate the carryover of dead cells or DNA, which may act as a template for the Templiphi reaction resulting in contamination of the sequencing reactions.
The unique combination of ethanol wash bath with halogen pin drying on Genetix colony pickers eliminates DNA carryover.
