Disposable flask-bioreactor for long-term, high level protein expression from adherent cells incorporates a woven polyethylene teraphthalate matrix in the cell compartment
Visitors to International Biotech 2003 (stand B500) at Olympia (London, UK) in November will have the chance to see and hear an introductory talk, on Integra Biosciences's new disposable flask-bioreactor for long-term, high level protein expression from adherent cells.
Designed specifically for protein expression from adherent cells the Celline adhere incorporates a woven polyethylene teraphthalate (PET) matrix in the cell compartment to provide an ideal surface for attachment of anchorage dependent HEK, BHK and CHO cells.
With achievable cell densities of between 107 and 108 cells per millilitre, Celline adhere provides cell concentrations typically two orders of magnitude higher than conventional static cell culture techniques.
Benefiting from these high cell densities, product concentrations achievable in Celline adhere are several milligrams per millilitre making the bioreactor highly suitable for protein expression in transfected cell lines even over extended periods of time.
Designed for ease of use the Celline bioreactor family utilises novel membrane technology to separate off the cultivation chamber with an upper semi-permeable membrane through which nutrient can diffuse and a lower one that allows gases to diffuse.
Separate ports allow selective access to the upper nutrient supply chamber and the central cultivation chamber.
This novel compartmentalised arrangement means that medium can be exchanged without influencing the function or growth of the cells and gases. Consequently, traditional cell growth limitations brought about by a lack of nutrients or the accumulation of metabolic waste can be overcome just as easily as oxygen deficiency.
The unique two-compartment design of Celline adhere makes bioreactor deficiencies in nutrition and oxygenation problems of the past.
