Three important international meetings chosen to launch novel disposable flask-bioreactor for long-term, high level protein expression from adherent cells
Integra Biosciences, a specialist in cell cultivation equipment, has chosen three important international meetings to launch the Celline Adhere, a novel disposable flask-bioreactor for long-term, high level protein expression from adherent cells.
Over the next month cell biologists visiting the 43rd American Society for Cell Biology (booth 1944),International Biotech 2003 (stand B500) and the 33rd Japanese Society of Immunology meeting (booths 82/83) will have the chance to see, review application posters and hear an introductory talk on the new product.
Optimised for protein expression from adherent cells the Celline adhere incorporates a woven polyethylene teraphthalate (Pet) matrix in the cell compartment to provide an ideal surface for attachment of anchorage dependent cells as for example HEK, BHK and CHO.
Designed for ease of use, the Celline Adhere utilises novel membrane technology to separate off the cultivation chamber with an upper semi-permeable membrane through which nutrient can diffuse and a lower one that allows gases to diffuse.
Separate ports allow selective access to the upper nutrient supply chamber and the central cultivation chamber.
This compartmentalised arrangement means that medium can be exchanged without influencing the function or growth of the cells.With achievable cell densities of between 107 and 108 cells per millilitre, Celline Adhere provides cell concentrations typically two orders of magnitude higher than conventional static cell culture techniques.
Benefiting from these high cell densities, product concentrations achievable in Celline adhere are several milligrams per millilitre making the bioreactor highly suitable for protein expression in transfected cell lines even over extended periods of time.
