Application study describes the advantages of a novel disposable flask bioreactor for long-term cultivation of high levels of recombinant proteins
Integra Biosciences has announced a new applications study, undertaken in conjunction with Eugenix Biotechnologies (Tagerwilen, Switzerland), describing the advantages of a novel disposable flask bioreactor for long-term cultivation of high levels of recombinant proteins.
Optimised for protein expression from adherent cells, the Celline Adhere disposable flask bioreactor incorporates a woven polyethylene teraphthalate matrix in the cell compartment to provide an ideal surface for attachment of anchorage dependent cells such as HEK, BHK and CHO. Through use of a novel membrane technology in a two-compartment bioreactor the Celline Adhere enables high cell densities and corresponding product concentrations to be grown in both batch and semi-continuous culture modes.
The study shows that up to 70 traditional cell culture flasks (75cm2) are necessary to achieve the same protein yields as a single Celline Adhere.
In addition the smaller volume of resultant concentrated protein product offers significant savings in the time, labour and material costs of the downstream processing and purification.
The study clearly demonstrates the utility of the Celline Adhere in rapidly and cost effectively generating protein lead structures from an adherent mammalian cell line in quantities of 50-200mg suitable for biochemical and in-vivo activity studies.
Further information on the Celline Adhere and a copy of the applications study may be obtained direct from Integra Biosciences
