New range of inverted microscopes has a number of unique design features specifically for demanding applications in fluorescence
The new IX2 range of inverted microscopes from Olympus has a number of unique design features specifically for demanding applications in fluorescence.
A key requirement in life science fluorescence applications is to achieve the strongest signal, while at the same time protecting the precious specimen from damage.
To achieve this result, users work with narrow bandpath filters, or multi-excitation filter combinations for the simultaneous observation of different labels.
Both techniques need the maximum possible light intensity.
The unique design of the new straight condenser provides the highest brightness available, resulting in best signal intensity with minimum exposure times.
The six cube turret maximises the flexibility for fluorescence work.
For the utmost flexibility in applications development, the optical bench system provides eight optical ports where secondary light sources and cameras can easily be connected.
Extra space is created by employing an L-shaped illuminator, reducing the necessary space for the microscope setup.
This allows the user to attach a confocal unit, camera system, or additional light source to the back ports of the microscope.
Morphological (eg DIC) and functional information (eg fluorescence) can be acquired in the same experiment, just by a simple change of the mirror unit from fluorescence observation to Nomarski DIC.
In addition, six position filter wheels can be mounted on the same system.
Modular motorised six position cube turret and filter wheels can be used for the automated and computer controlled acquisition of images.
Additional motorised components like a transmitted light condenser, reflected/ transmitted light shutters, and Z-drive extend the set up for multi-dimensional techniques and deconvolution.
Single molecule detection and observation of biological processes at the cell level can be obtained by total internal reflection fluorescence microscopy (TIRFM).
Laser illumination is reflected from the surface, rather than penetrating the specimen deeply and causing background fluorescence.
Exclusive high NA objectives and a reflected illuminator are designed for this application.
The IX2 frame provides the high performance optics, and superior stability required for fluorescence research.
