Capillary electrophoresis instrument can be used for performing high throughput DNA fragment sizing analysis, eliminating some tedious slab gel work
The ability to separate dsDNA fragments in the 100bp-3000bp size range is now available on the cePro 9600 system from CombiSep. "There is an expressed demand for automated analytical instruments that eliminate tedious slab gel work", says Dennis Tallman, CombiSep VP of sales and marketing.
CombiSep reports that the application has demonstrated that the cePro 9600 can be used for performing high throughput DNA fragment sizing analysis.
A simple, robust desalting procedure has been developed which facilitates the analysis of DNA fragments initially present in salt matrices (eg PCR products).
The method has been shown to provide high resolution across a wide sizing range with good detection sensitivity.
When internal standards are employed, this approach can yield accurate sizing and quantification of DNA fragments with high throughput and increased automation compared to conventional slab gel electrophoresis methods.
CombiSep manufactures high throughput separation systems for the pharmaceutical, biotechnology and chemical industries to increase productivity in discovery, development and quality control processes.
The cePro 9600 is a multiplexed capillary electrophoresis (CE) instrument providing near universal UV absorbance detection and the capability to perform 96 CE separations in parallel, dramatically increasing sample throughput.
The cePro 9600 is available in the UK from Presearch, and the application note can be downloaded from Presearch's website.
