Instrument gains its exceptional sensitivity and flexibility in combination with unprecedented ease of use from a unique fusion of miniaturised optics and electronics
PicoQuant has released a new version of its MicroTime data analysis software.
The MicroTime 200 fluorescence microscope system is a powerful newly developed instrument capable of fluorescence lifetime imaging (Flim) and single molecule detection (SMD).
It contains the complete optics and electronics for recording virtually all aspects of the fluorescence dynamics of microscopic samples including single molecules.
The instrument gains its exceptional sensitivity and flexibility in combination with unprecedented ease of use from a unique fusion of miniaturised state-of-the-art optics and electronics.
This allows an instrument of comparable complexity and power to be run in routine work, yet without having to spend more time on instrument maintenance than on original scientific content.
With the microscope, sub-micrometer spatial and picosecond time resolution can be achieved.
The instrument's time-tagged time-resolved (TTTR) measurement mode allows it to perform vastly different measurement tasks based on one single data format, yet without any sacrifice of information available from each single photon. This in turn allows handling all measurement data in a standardised and yet flexible way.
Based on this powerful concept, the new data analysis software of the MicroTime 200 is designed for integration of virtually all algorithms and methods for the analysis of fluorescence dynamics users may require. Based on a single TTTR data collection run, users can perform an unlimited number of analysis steps without losing track of interdependence and origin of their measurement and analysis data.
All this is realised through a workspace concept, in which the TTTR file is the origin of each measurement.
Derived results can be obtained through a vast set of analysis tools, such as intensity time trace, burst analysis, lifetime histogramming, fluorescence correlation spectroscopy (FCS), lifetime imaging and fluorescence resonance energy transfer (Fret).
All derived data is maintained in the workspaces, including a log file keeping track of all measurement and analysis steps.
Images can be further manipulated through a built-in image calculator.
For off-line data analysis the MicroTime software can even be used separate from the microscope, on a different PC.
