New technical note aimed at laboratory managers, especially in the pharmaceutical industry, covers preheating requirements in high temperature liquid chromatography
Extended temperature separations specialist Selerity Technologies has produced a new free technical note detailing preheating requirements in high temperature liquid chromatography (HTLC). The new technical note is aimed at laboratory managers, especially in the pharmaceutical industry.
It is intended to introduce readers to the performance benefits accrued from pre-heating of the mobile phase in high temperature liquid chromatography.
The note describes how preheating of the mobile phase is significant in attaining maximum performance in HTLC, since the relationship between temperature and viscosity strongly influences the mobile phase flow profile and the diffusion characteristics.
A new highly responsive low-volume, low-mass solvent preheater has been designed by Selerity to keep the amount of tubing required for preheating the mobile phase low, and without invading the fluid path.
With preheating, peaks are sharper and resolution is greatly improved.
This highly responsive preheater design makes temperature programming possible with the most commonly used chromatographic columns, without loss of efficiency.
The note describes the flow rates necessary to bring the mobile phase to the temperature of the separation column.
The technical note is available free of charge via email.
