Sigma has introduced a new plasmid DNA isolation method designed to help researchers achieve high-quality DNA for automated sequencing in less time
The GenElute five-minute plasmid miniprep kit procedure relies on two new technologies developed at Sigma.
The first is a patent-pending, novel lysis chemistry that enables DNA purification directly from an overnight culture.
The standard miniprep steps of pelleting cells and neutralising and clearing the lysate have been eliminated.
The second is a unique DNA binding column, specially designed to isolate plasmid DNA directly from bacterial growth media.
The combination of these discoveries has resulted in an ultra-streamlined procedure that does not compromise DNA quality.
"The new GenElute five-minute plasmid miniprep kit is a great example of the type of innovations that are possible with Sigma's silica-binding chemistry," said Keith Jolliff, global marketing manager of molecular biology products.
"We focused on researchers conducting minipreps specifically for DNA sequencing and revised the prep time and yield accordingly.
"With the development of our novel lysis buffer coupled with an excellent silica-binding chemistry, we reduced prep time to around five minutes while still delivering enough DNA for around 15 sequencing reactions."
