Whole genome amplification kit amplifies nanogram amounts of starting DNA into microgram yields in less than three hours, regardless of source
Sigma has launched a new whole genome amplification kit, GenomePlex whole genome amplification.
In October 2004, Sigma entered into an exclusive licencing agreement with Rubicon Genomics to further develop and commercialise the GenomePlex WGA technology.
The GenomePlex whole genome amplification (WGA) kit utilises a proprietary amplification method designed for robust and accurate amplification of limited source DNA.
Sigma says that, in less than three hours, GenomePlex WGA successfully amplifies nanogram amounts of starting DNA, regardless of source, into microgram yields.
The new GenomePlex WGA kit is sufficient for 50 reactions and recommended to be used with Sigma's JumpStart Taq DNA polymerase.
Additions to the whole genome amplification product line for 2005 are expected to include a kit sufficient for 100 reactions and a kit with JumpStart Taq DNA polymerase included.
Advantages claimed for GenomePlex WGA include:.
Flexibility to study DNA from any source.
No detectable locus or allele bias.
Compatibility with a variety of microarray, capillary, and homogenous platforms for sequencing, genotyping, CGH, Fish, ChIP, forensics, and biosurveillance.
Increased sensitivity and accuracy for population studies, mutation discovery, and pharmacogenomics.
Robust amplification of problematic and highly degraded DNA from formalin-fixed, serum, buccal swab, archived, forensic and environmental samples. Substantially faster than other WGA methods.
"The GenomePlex technology offers an efficient and economical approach to whole genome amplification," commented Keith Jolliff, global marketing manager of molecular biology at Sigma-Aldrich.
"We are very excited about the GenomePlex technology and feel it is an excellent complement to our existing nucleic acid amplification product line as well as our oligonucleotide offering through Sigma-Genosys.
"And we are already developing the next generation of GenomePlex products, fully expecting to introduce two new kits with one sufficient for 100 reactions and the other with JumpStart Taq DNA Polymerase included in early 2005."
