Syngene has introduced its GeneTools MADGE system, a robot compatible software designed to make high throughput gel studies both quicker and easier to perform
Syngene has introduced its GeneTools MADGE system, a robot compatible software designed to make high throughput gel studies both quicker and easier to perform.
Microtitre Array Diagonal Gel Electrophoresis (MADGE) gels are run in a grid format based on a micro-titre plate and have the potential for up to 768 tracks within a single gel.
Therefore, using GeneTools MADGE software to automate this type of analysis will save users many hours of repetitive manual gel comparisons.
With GeneTools MADGE, users can select the degree of rotation and electrophoresis direction, as well as the number of 96 well data sets using columns and rows.
The easy-to-use grid allows manual realignment of track positions, making track location quick and simple.
Once all tracks are locked in place, the software automatically detects all peaks in a horizontally aligned view.
In the aligned view, marker tracks (standards) can be selected and their peaks assigned, enabling calculation of molecular weight for all unknown peaks in the gel.
Matching to a standard track may also be carried out, based on molecular weight, Rf or position from the top of a track.
Results are exportable to Excel* which, using a simple formula, can rapidly convert the figures into genotype information.
The software even allows report generation within the package or through Word*.
Paul Ellwood, Syngene's Divisional Sales and Marketing Director said: "With MADGE gels, peaks often migrate at different rates across and down the gels leading to the need for a series of track alignment steps. The feedback we have had on GeneTools MADGE is that it can easily cope with realignment and is superior to other packages in analysing peaks, even when the gels have been run with more than 96 tracks.
This makes it unique among MADGE analysis systems and will greatly benefit molecular biologists involved in structural genomics, DNA arrays or gene expression research."
