Chemiluminescent image analysis system is being used at the University of Sheffield to speed up research into the causes of blood vessel leakage
Syngene reports that its ChemiGenius2 chemiluminescent image analysis system is being used at the University of Sheffield, a highly ranked centre for biological sciences, to speed up research into the causes of blood vessel leakage.
Researchers at Sheffield are using the ChemiGenius2 to analyse the amount of a Tie receptor present on chemiluminescent Western blots stained with SuperSignal.
Since the Tie receptor has been implicated in helping prevent blood vessel injury during inflammatory responses, the researchers are hoping that information on this and other receptors will lead to therapies to control life threatening blood vessel leaks following coronary bypass surgery. Marie Marron, a research fellow in the division of clinical sciences at the university, said: "Prior to using a ChemiGenius2, we exposed our blots to film, scanned in the film to PC, and then applied software analysis to produce the raw densitometry data.
"This was very time consuming because for each antibody we use to detect the Tie receptor, we had to optimise the exposure conditions, which meant using a large number of films to get a correctly exposed image.
"With the ChemiGenius2 it is much quicker since the system detects if the blot is over saturated, so you only keep the images you want. "Also the quantification and analysis of the immunoblot is easier as you can transfer the densitometric data straight into an Excel spreadsheet for further analysis".
Laura Sullivan, Syngene's divisional manager, commented: "We are delighted to see a major university using the ChemiGenius2 to speed up the chemiluminescent research part of a project to help unravel the mysteries of blood vessel leakage.
"Their work could be translated to the clinical setting, which is why using the ChemiGenius2 for obtaining reproducible results that can be captured, recorded and analysed to GLP standards is so important."
